In vivo Effectivity of Collagen Extract from Clarias gariepinus (Burchell, 1822) ‘Sangkuriang’ on Formation of Malondialdehyde and Macrophages in Burn Healing: Effectivity of Collagen Extract from Clarias gariepinus in Burn Healing

Ary Andini; Retno  Handajani; Soetjipto  Koesnowidagdo; Miranda  Zawazi Ichsan; Luciano  Pighinelli

In vivo Effectivity of Collagen Extract from Clarias gariepinus (Burchell, 1822) ‘Sangkuriang’ on Formation of Malondialdehyde and Macrophages in Burn Healing

Effectivity of Collagen Extract from Clarias gariepinus in Burn Healing

Authors

Ary Andini Faculty of Health, Nahdlatul Ulama University of Surabaya, Jl. Jemursari No. 51-57, Surabaya 60237, Indonesia
Retno Handajani Departement of Biochemistry, Faculty of Medicine, Airlangga University, Jl. Mayjen Prof. Dr. Moestopo No. 47, Surabaya 60132, Indonesia
Soetjipto Koesnowidagdo Departement of Biochemistry, Faculty of Medicine, Airlangga University, Jl. Mayjen Prof. Dr. Moestopo No. 47, Surabaya 60132, Indonesia
Miranda Zawazi Ichsan College of Bioengineering, Chongqing University, No. 174 Shazheng Street, Shapingba District, Chongqing 400044, People’s Republic of China
Luciano Pighinelli Biomatter P&D and Innovation Biomaterials Lab. Incubadora Empresarial do Centro de Biotecnologia – IECBiot Av. Bento Gonçalves, 9500, Prédio 43421 - Setor IV Campus do Vale da UFRGS, Bairro Agronomia, CEP 91501-970, Porto Alegre – RS, Brazil

Keywords:

Burn injury, Catfish, Collagen extract, Skin, Wound healing

Abstract

Topical collagen on skin wound gift moist state that could stimulate reepithelization, proliferation, and epithelial cell migration to conduct growth factor activity on the wound area. One of the sources of collagen is Sangkuriang catfish [Clarias gariepinus (Burchell, 1822) ‘Sangkuriang’] skin that had low immunoreactivity in the human body. This study aimed to determine the influence of topical treatment with Sangkuriang catfish skin extract on malondialdehyde serum and macrophage total on burn injury. Collagen of Sangkuriang catfish skin was extracted by HCl 2 % for 48 h and neutralized by NaOH 1 M. Sangkuriang catfish skin extract contained amino acid of collagen that could repair and stimulate skin cell. This study was an experimental study that used 24 male Rattus norvegicus strain Wistar were divided into K3, P3, K10, and P10 groups. K group was treated with aquadest topical as a burn healing treatment for 3 d (K3) and 10 d (K10). The P group was treated with topical collagen extract as a burn
healing treatment for 3 d (P3) and 10 d (P10). The results showed that treatment by collagen from Sangkuriang catfish skin could not decrease MDA serum on 3rd d and 10 d because of P > 0.05 (P = 0.713). Furthermore, it could not decrease macrophage total on 3rd d (P = 0.427) but significantly decrease to 10 d for K10 and P10. Collagen topical from Sangkuriang catfish could accelerate burn to heal by decreasing macrophages total on 10th d due to P < 0.05 (P = 0.000).

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LS-576

Published

2020-09-06

How to Cite

Andini, A., Handajani, R. ., Koesnowidagdo, S., Zawazi Ichsan, M. ., & Pighinelli, L. (2020). In vivo Effectivity of Collagen Extract from Clarias gariepinus (Burchell, 1822) ‘Sangkuriang’ on Formation of Malondialdehyde and Macrophages in Burn Healing: Effectivity of Collagen Extract from Clarias gariepinus in Burn Healing. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 57(3), 35–40. Retrieved from https://www.ppaspk.org/index.php/PPAS-B/article/view/5

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Issue

Vol. 57 No. 3 (2020)

Section

Research Articles

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