Catalase Activity as a Bio-Indicator of Lead+Nickel Toxicity in Carnivorous Fish, Channa striata
Response of CAT activity in fish
Keywords:
Carnivorous fish, Chronic exposure, Metals mixture, Antioxidant enzyme, Channa striataAbstract
The freshwater ecosystems are extensively polluted with heavy metals discharged from industrial, domestic,
and other human activities. These metals are significant stimulators of oxidative stress in aquatic organisms, especially fish, leading to the creation of reactive oxygen species. Therefore, current research was conducted to elucidate the toxic effect of heavy metals mixture (Pb+Ni) on catalase activity in various organs (gills, liver, kidney, brain, muscle and heart) of carnivorous fish, Channa striata exposed to sub-lethal concentrations (1/3rd, 1/4th and 1/5th of LC50) for a period of 14-day. Fish were sampled for enzyme study after 7 and 14-days. Results showed that all concentrations of metals mixture significantly decreased the CAT activity in selected organs of the fish however, maximum depletion was observed in 1/3rd of LC50 concentration followed by the order of 1/4th>1/5th. The CAT activity was decreased with increasing the concentration and duration of exposure.
References
Vutukuru, S.S., C. Suma, K.R. Madhavi, J.S. Pauleena, J.V. Rao & Y. Anjaneyulu. Studies on the development of potential biomarkers for rapid assessment of copper toxicity to freshwater fish using Esomus danricus as model. International Journal of Environmental Research and Public Health 2: 63-73 (2005).
Rauf, A., M. Javed, M. Ubaidullah & S. Abdullah. Heavy metal levels in three major carps (Catlacatla, Labeo rohita and Cirrhina mirgala) from the river Ravi, Pakistan. Pakistan Veterinary Journal 29: 24-26 (2009).
Fent, K. Studies on the effects of aquatic pollution on fish. Life Science Journal 10: 637-642 (2007).
Censi, P., S.E. Spoto, F. Saiano, M. Sprovieri, S.Mazzola, G. Nardone, S.I. Di-Geronimo, R. Punturo and D. Ottonello. Heavy metals in coastal water systems. A case study from the northwestern Gulf of Thailand. Chemosphere 64: 1167-1176 (2006).
Jabeen, G., M. Javed & H. Azmat. Assessment of heavy metals in the fish collected from the river Ravi, Pakistan. Pakistan Veterinary Journal 32: 107– 111(2012).
Van-dar-Oost, R., J. Beyer & N.P.E. Vermeulen. Fish bioaccumulation and biomarkers in environment risk assessment. Environmental Toxicology and Pharmacology 13: 57-149 (2003).
Rauf, A., M. Javed & U. Ullah. Heavy metals level in three major carps (Catla catla, Labeo rohita and Cirrhina mrigala) from the river Ravi, Pakistan. Pakistan. Veterinary Journal 29: 24-26(2009).
Jabeen, G. & M. Javed. Evaluation of arsenic toxicity to biota in river Ravi (Pakistan) aquatic ecosystem. International Journal of Agriculture Biology 13: 929-934 (2011).
Palaniappan, P.L.R.M. & S. Karthikeyan. Bioaccumulation and depuration of chromium in the selected organs and whole body tissues of freshwater fish Cirrhinus mrigala individually and in binary solutions with nickel. Journal of Environmental Sciences 21: 229-236 (2009).
Coen, N., C. Mothersill, M. Kadhim, & E.G. Wright. Heavy metals of relevance to human health induce genomic instability. Pathology 195: 293-299 (2001).
Sfakianakis, D.G., E. Renieri, M. Kentouri & A.M.Tsatsakis. Effect of heavy metals on fish larvae deformities. Environmental Research 137: 246-255(2015).
Creti, P., F. Trinchella & R. Scudiero. Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems. Environmental Monitoring and Assessment 165: 321-329 (2010).
Jezierska, B. & M. Witeska. 2001. Summary of metal-induced disturbances in fish organism. In: Metal Toxicity to Fish. Veterinarni Medicina 56: 537-546 (2001).
Ercal, N., H. Gurer-Orhan & N. Aykin-Burns. Toxic metals and oxidative stress Part I: Mechanisms involved in metal-induced oxidative damage.Current Topics in Medicinal Chemistry 1: 529-539(2001).
Winzer, K., W. Becker., C.J.F. Van-Noorden & A.Kohler. Short time induction of oxidative stress in hepatocytes of the European flounder (Platichthys flesus). Marine Environmental Research 50:495-501(2000).
Mates, J.M., C. Perez-Gomez & C.I. Nunezde. Antioxidant enzymes and human diseases. Clinical Biochemistry 32: 595-603 (1999).
Sarwar, U., T. Ahmed, S. Saeed, S. Abdullah, M.Javed, K. Abbas & M.A. Zia. 2014. Purification and partial characterization of liver catalase enzyme from controlled and Pb+Cd metal mixture stressed grass carp (Ctenopharyngodon idella). Pakistan Journal of Zoology 48:1733-1740.
Faheem, M., A. Qayyum, K. Sulehria, M. Tariq, I.Khadija, A. Fiaz & M. Saeed. Effect of sub-lethal dose of cadmium chloride on biochemical profile and catalase activity in freshwater fish Oreochromis niloticus. Biologia 58: 73-78 (2012).
Atli, G. & M. Canli. Enzymatic responses to metal exposures in a freshwater fish Oreochromis niloticus. Comparative Biochemistry and Physiology C 145: 282-287 (2007).
Atli, G. & M. Canli. Response of antioxidant system of freshwater fish Oreochromis niloticus to acute and chronic metal (Cd, Cu, Cr, Zn and Fe) exposures. Ecotoxicology and Environmental Safety 73: 1884-1889 (2010).
Jezierska, B. & P. Sarnowski. The effects of mercury, copper and cadmium during single and combined exposure on oxygen consumption of Oncorhynchus mykiss and Cyprinus carpio(L.) larvae. Archives in Polish Fish 10:15-22 (2002).
Anum, S., S. Abdullah, K. Abbas, H. Naz &W. Hassan. Changes in antioxidant enzyme (glutathione S-transferase) activity in fish Channa striata exposed to the different aquatic pollutants (heavy metals and pesticides mixture). 6th International Fisheries Symposium (2017).
A.P.H.A. (20th Ed) Standard Methods for the Examination of Water and Wastewater, American Public Health Association, New York (1998).
Chance, M. and A.C. Maehly. Assay of catalase and peroxidase. Methods Enzymology 2: 764-817(1995).
Stohs, S.J. & D. Bagchi. Oxidative mechanisms in the toxicity of metals ions. Free Radical Biology and Medicine 2: 321–336 (1995).
Pinto, E., T.C.S. Sigaud-Kutner, M.A.S. Leitao, O.K. Okamoto, D. Morse & P. Colepicolo. Heavy metal-induced oxidative stress in algae. Journal of Phycology 39:1008-1018 (2003).
Valko, M., H. Morris & M.T.D. Cronin. Metals, toxicity and oxidative stress. Current Medicinal Chemistry 12: 1161–1208 (2005).
Lushchak, V.I. Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicology 1: 13-30 (2011).
Martinez-Alvares, R.M., A.E. Morales &A. Sanz. Antioxidant defenses in fish: Biotic and abiotic factors. Reviews in Fish Biology and Fisheries 15: 75-88 (2005).
Atli, G. &M. Canli. Enzymatic responses to metal exposures in a freshwater fish Oreochromis niloticus. Comparative Biochemistry and Physiology 45: 282-287(2008).
Tripathi B.N., S.K. Mehta, A. Amar & G.P. Gaur.Oxidative stress in Scenedesmus sp. during short- and long-term exposure to Cu2+ and
Zn2+. Chemosphere 62:538-44(2006).
Firat, O. & F. Kargin. Effects of zinc and cadmium on erythrocyte antioxidant systems of fresh water fish Oreochromis niloticus. Journal of biochemical and molecular Toxicology 24: 223-229 (2010).
Farombi, E.O., O.A. Adelowo & Y.R. Ajimoko. Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigeria Ogun River. International Journal of Environmental Research and Public Health 4: 158-165 (2007).
Madhavan, P. & K. Elumalai. Effects of chromium (VI) on the lipid peroxidation and antioxidant parameterzs in the gill and kidney tissues of catfish, Clarias batrachus (Linnaeus, 1758) (Actinopterygii: Siluriformes). International Journal of Advanced
Research in Biological Sciences3: 249-255 (2016).
Velma, V. & P.B. Tchounwou. Chromium-induced biochemical, genotoxic and histopathologic effects in liver and kidney of goldfish, Carassius auratus. Mutation Research 698: 43-51(2010).
Shen, J., W. Zhang, J. Wu &Y. Zhu. The synergistic reversal effect of multidrug resistance by quercetin and hyperthermia in doxorubicin-resistant human myelogenous leukemia cells. International Journal of Hyperthermia 24: 151–159 (2008).
Kubrak, O.I., O.V. Lushchak, J.V. Lushchak, I.M. Torous, J.M. Storey, K.B. Storey & V.I. Lushchak.Chromium effects on free radical processes in goldfish tissues: Comparison of Cr (III) and Cr (VI) exposures on oxidative stress markers, glutathione status and antioxidant enzymes. Comparative Biochemistry and Physiology, 152: 360-370 (2010).
Vasylkiv O.Y., O.I. Kubrak, K.B. Storey & V.I. Lushchak. Cytotoxicity of chromium ions may be connected with induction of oxidative stress. Chemosphere 80: 1044-1049 (2010).
Faheem, M., A. Qayyum, K. Sulehria, M. Tariq, I. Khadija, A. Fiaz & M. Saeed. Effect of sub-lethal dose of cadmium chloride on biochemical profile and catalase activity in fresh water fish Oreochromis niloticus. Biologia, 58: 73-78 (2012).
Saliu, J.K. & K.A. Bawa-Allah. Toxicological effects of lead and zinc on the antioxidant enzyme activities of post juvenile Clarias gariepinus. Resources and Environment 2: 21-26 (2012).
Yilmaz, H.R., Y. Turkoz, E. Yksel & I. Orun. An investigation of antioxidant enzymes activities in liver of Cyprinus carpio taken from different stations in the karakaya dam lake. International Journal of Science and Technology 1: 1-6 (2006).
Romeo, M., N. Bennani, M. Gnassia-Barelli, M. Lafaurie & J.P. Girard. Cadmium and copper display different responses towards oxidative stress in the kidney of the sea bass Dicentrarchus labrax. Aquatic Toxicology 48: 185-194 (2000).
Sunaina & B.A. Ansari. Biochemical markers of oxidative stress in zebra fish Danio rerio exposed to cadmium chloride. Annals of Biological Research 6:6-129 (2015).
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