Effect of Humic Acid Levels on the Production of Gladiolus Cultivars

Authors

  • Ahmad Naeem Department of Horticulture, Faculty of Crop Production Sciences, The University of Agriculture Peshawar, 25120, Pakistan
  • Noor Ul Amin Department of Horticulture, Faculty of Crop Production Sciences, The University of Agriculture Peshawar, 25120, Pakistan
  • Hamza Ali State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Masood Ahmad Department of Horticulture, Faculty of Crop Production Sciences, The University of Agriculture Peshawar, 25120, Pakistan
  • Abdul Mateen Khattak Department of Horticulture, Faculty of Crop Production Sciences, The University of Agriculture Peshawar, 25120, Pakistan
  • Amna Shafi Agriculture Research Institute Tarnab, Peshawar, Pakistan
  • Ateeq Ur Rehman Department of Horticulture, Faculty of Crop Production Sciences, The University of Agriculture Peshawar, 25120, Pakistan
  • Habib Ur Rehman Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan

DOI:

https://doi.org/10.53560/PPASB(62-4)1121

Keywords:

Bulbous Crops, Flowering, Gladiolus, Humic Acid, Vase Life

Abstract

Gladiolus is a valuable ornamental crop, widely cultivated for its aesthetic appeal and commercial demand. However, its growth and flowering performance are often hindered by poor soil fertility and suboptimal nutrient management, which needs to be optimized for its commercial production under the agroclimatic conditions of Peshawar, Pakistan. Humic acid is a natural organic substance and is known to enhance soil properties and improve plant growth. Therefore, this experiment was conducted under RCBD split plot arrangement to evaluate the different humic acid levels and their influence on five different gladiolus cultivars. Results indicated that both humic acid and cultivars significantly influenced vegetative and reproductive attributes. The humic acid at the rate of 4 kg ha⁻¹ treatment was most effective, resulting in improved emergence, number of leaves, leaf area, spike emergence, 1st floret opening, florets per spike, field flower persistence, vase life, corm weight, and number of cormels. Enhanced performance was attributed to better nutrient uptake, chlorophyll synthesis, hormonal balance, and source–sink relationships under humic acid supplementation. Among cultivars, ‘White Prosperity’ showed superior vegetative growth and cormel production, while ‘Priscilla’ had longer vase life and heavier corms, indicating genetic differences in nutrient use and reproductive efficiency. The results show that humic acid not only promotes vegetative growth but also accelerates floral initiation, increases floret production, delays senescence, and enhances postharvest quality. It is concluded that humic acid at the rate of 4 kg ha⁻¹, in cultivars White Prosperity and Priscilla, was optimal for commercial gladiolus cultivation under Peshawar’s agro-climatic conditions.

References

1. M. Kumar, V. Chaudhary, U. Sirohi, J. Singh, M.K. Yadav, S. Prakash, A. Kumar, V. Kumar, V. Pal, C. Chauhan, and K. Kaushik. In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): a systematic review analysis based on more than 50 years Research. Horticulturae 10(2): 148 (2024).

2. A. Javaid, R.K. Pandey, A.H. Shah, P. Bakshi, I.T. Nazki, N. Kaushal, G. Chand, S. Dogra, R. Kumar, A.K. Singh, and A. Singh. Response of Gladiolus grandiflorus varieties to planting date: effects on growth, flowering, and vase life. BMC Plant Biology 25(1): 481 (2025).

3. M. Bala and F. Sala. Comparative analysis of some Gladiolus varieties in relation to vegetative indices and floral quality parameters. Scientific Papers Series Management, Economic Engineering in Agriculture and Rural Development 21(3): 159-166. (2021).

4. N. Devrani, P. Kakkar, A. Sahu, and C. Tiwari. Global trends in floriculture. In: Floriculture and Landscaping Chronicles: A Collaborative Insights. A. Burud, S.M. Kolur, D.R. Karthik, D.L. Kumar, D. Yadav, and N. Kaushal (Eds.) Stella International Publication, Haryana, India pp. 190-221 (2023).

5. M. Ahmad and A. Rab. Calcium effects on post-harvest attributes and vase life of gladiolus using different methods of application. Pakistan Journal of Botany 52(1):167-179 (2020).

6. K. Ampong, M.S. Thilakaranthna, and L.Y. Gorim. Understanding the role of humic acids on crop performance and soil health. Frontiers in Agronomy 4: 848621 (2022).

7. R.E. Pettit. Organic matter, humus, humate, humic acid, fulvic acid and humin: their importance in soil fertility and plant health. CTI Research 10: 1-7 (2004). https://earthwiseagriculture.net/wp-content/uploads/2018/02/ORGANICMATTERPettit.pdf

8. F. Nabi, A. Sarfaraz, R. Kama, R. Kanwal, and H. Li. Structure-based function of humic acid in abiotic stress alleviation in plants: a review. Plants 14(13): 1916 (2025).

9. J. Tiwari, A.L. Ramanathan, K. Bauddh, and J. Korstad. Humic substances: Structure, function and benefits for agroecosystems-A review. Pedosphere 33(2): 237-249 (2023).

10. Q. Chen, Z. Qu, G. Ma, W. Wang, J. Dai, M. Zhang, Z. Wei, and Z. Liu. Humic acid modulates growth, photosynthesis, hormone and osmolytes system of maize under drought conditions. Agricultural Water Management 263:107447 (2022).

11. S. Nasir, M. Ahmad, H. Ali, S. Jabin, A. Khan, M. Ullah, A. Bano, H.A. Khan, A. Khan, and A. Ullah. Performance of Gladiolus Cultivars on Morphological Traits and Corm Production under the Agro-Climatic Conditions of Peshawar-Pakistan. Indus Journal of Bioscience Research 3(1): 58-63 (2025).

12. H. Ali, M. Ahmad, S. Jabin, R.Z. Muqarrab, I. Ahmad, M.A. Khan, M. Khan, I. Khalil, A. Basit, M. Kamal, and M. Ahmad. Influence of willow bark extracts and application times on the production of Roselle. Plant Protection 8(4): 671-677 (2024).

13. R.S. Nada, M.N. Soliman, M.M. Zarad, M.H. Sheta, S. Ullah, A.I. Abdel-Gawad, A.H. Ghoneim, and A.A. Elateeq. Effect of Organic Fertilizer and Plant Growth-Promoting Microbes on Growth, Flowering, and Oleanolic Acid Content in Calendula officinalis under Greenhouse Conditions. Egyptian Journal of Soil Science 64(3): 815-831 (2024).

14. M. Hassan, S.A. Shaaban, R.A. El Ziat, and K.A. Khaled. Laser-induced changes in the gene expression, growth and development of gladiolus grandiflorus cv.“White prosperity”. Scientific Reports 14(1): 6257 (2024).

15. K. Decsi, M. Ahmed, R. Rizk, D. Abdul-Hamid, and Z. Tóth. Analysis of plant physiological parameters and gene transcriptional changes under the influence of humic acid and humic acid–amino acid combinations in maize. International Journal of Molecular Sciences 25(24): 13280 (2024).

16. P. Bhatt and V.K. Singh. Effect of humic acid on soil properties and crop production–A review. Indian Journal of Agricultural Sciences 92(12): 1423-1430 (2022).

17. R. Motzo and F. Giunta. The effect of breeding on the phenology of Italian durum wheats: from landraces to modern cultivars. European Journal of Agronomy 26(4) 462-470 (2007).

18. M. Rizwan, M. Ahmad, A.M. Khattak, A. Mohammad, F. Ali, G. Nabi, S. Ayaz, and N. Ayaz. Influence of organic potash on the vegetative attributes and corm production of gladiolus cultivars. Bioscience Research 18(4): 3326-3333. (2021).

19. K.S. Karthika, I. Rashmi, and M.S. Parvathi. Biological functions, uptake and transport of essential nutrients in relation to plant growth. In: Plant Nutrients and Abiotic Stress Tolerance. M. Hasanuzzaman, M. Fujita, K. Nahar, B. Hawrylak-Nowak, and H. Oku (Eds.). Springer Nature Singapore pp. 1-49. (2018).

20. H.M. Fan, X.W. Wang, X. Sun, Y.Y. Li, X.Z. Sun, and C.S. Zheng. Effects of humic acid derived from sediments on growth, photosynthesis and chloroplast ultrastructure in chrysanthemum. Scientia Horticulturae 177: 118-123 (2014).

21. J.A.H. Murray, A. Jones, C. Godin, and J. Traas. Systems analysis of shoot apical meristem growth and development: integrating hormonal and mechanical signaling. The Plant Cell 24(10): 3907-3919 (2012).

22. M.A. Baldotto and L.E.B. Baldotto. Gladiolus development in response to bulb treatment with different concentrations of humic acids. Revista Ceres 60: 138-142 (2013).

23. Z. Rengel and P.M. Damon. Crops and genotypes differ in efficiency of potassium uptake and use. Physiologia Plantarum 133(4): 624-636 (2008).

24. S.K. Verma, P.K. Sahu, K. Kumar, G. Pal, S.K. Gond, R.N. Kharwar, and J.F. White. Endophyte roles in nutrient acquisition, root system architecture development and oxidative stress tolerance. Journal of Applied Microbiology 131(5): 2161-2177 (2021).

25. A.C. Souza, F.L. Olivares, L.E.P. Peres, A. Piccolo, and L.P. Canellas. Plant hormone crosstalk mediated by humic acids. Chemical and Biological Technologies in Agriculture 9(1): 29 (2022).

26. M. Babarabie, H. Zarei, S. Badeli, A. Danyaei, and F. Ghobadi. Humic acid and folic acid application improve marketable traits of cut tuberose (Polianthes tuberosa). Journal of Plant Physiology and Breeding 10(1): 85-91 (2020).

27. S. Proietti, V. Scariot, S. De Pascale, and R. Paradiso. Flowering mechanisms and environmental stimuli for flower transition: bases for production scheduling in greenhouse floriculture. Plants 11(3): 432 (2022).

28. S.Q. Zhang, L. Yuan, Z.A. Lin, Y.T. Li, S.W. Hu, and B.Q. Zhao. Advances in humic acid for promoting plant growth and its mechanism. Journal of Plant Nutrition and Fertilizers 23(4): 1065-1076 (2017).

29. A. El-Naggar and G.F.M. Imhmd. Influence of humic acid and mineral nutrition on the growth, yield of flowers and the chemical constituents of Rosa hybrida L. Scientific Journal of Flowers and Ornamental Plants 10(1): 17-26 (2023).

30. O.A. Ali. Role of humic substances and compost tea in improvement of endogenous hormones content, flowering and yield and its components of faba bean (Vicia faba L.). Annals of Agricultural Science, Moshtohor 53(3): 373-384 (2015).

31. A. Raza, A. Razzaq, S.S. Mehmood, X. Zou, X. Zhang, Y. Lv, and J. Xu. Impact of climate change on crops adaptation and strategies to tackle its outcome: a review. Plants 8(2): 34 (2019).

32. M.S. Elmongy, X. Wang, H. Zhou, and Y. Xia. Humic acid and auxins induced metabolic changes and differential gene expression during adventitious root development in azalea microshoots. HortScience 55(6): 926-935 (2020).

33. S.M. McKim. How plants grow up. Journal of Integrative Plant Biology 61(3): 257-277 (2019).

34. F.G. Rahbar, A. Vaziri, M.H. Asil, S.T. Sasani, and J. Olfati. Effects of humic acid on antioxidant defense system and senescence-related gene expression in leaves of longiflorum × asiatic lilies (LA lilium hybrid). Journal of Soil Science and Plant Nutrition 23(3): 3500-3507 (2023).

35. H.M. Fan, T. Li, X. Sun, X.Z. Sun, and C.S. Zheng. Effects of humic acid derived from sediments on the postharvest vase life extension in cut chrysanthemum flowers. Postharvest Biology and Technology 101: 82-87 (2015).

36. T. Zhou, X. Qiu, L. Zhao, W. Yang, F. Wen, Q. Wu, and J. Pei. Optimal light intensity and quality increased the saffron daughter corm yield by inhibiting the degradation of reserves in mother corms during the reproductive stage. Industrial Crops and Products 176: 114396 (2022).

37. W.H. Mohamed. Effects of humic acid and calcium forms on dry weight and nutrient uptake of maize plant under saline condition. Australian Journal of Basic and Applied Sciences 6(8): 597-604 (2012).

38. M.A. Shajari, P.R. Moghaddam, R. Ghorbani, and A. Koocheki. Increasing saffron (Crocus sativus L.) corm size through the mycorrhizal inoculation, humic acid application and irrigation managements. Journal of Plant Nutrition 41(8): 1047-1064 (2018).

39. R. Sarje, A.K. Abhangrao, S. Jayakumar, R. Gupta, S. Pathania, and B.V. Sree. Effect of pre and postharvest factors on vase life of gladiolus or effect of different floral preservatives on vase life of gladiolus. International Journal of Plant & Soil Science 36(7): 297-303 (2024).

40. M. Hamad and M. Tantawy. Effect of different humic acids sources on the plant growth, calcium and iron utilization by sorghum. Egyptian Journal of Soil Science 58(3): 291-307 (2018).

41. M. Ahmad and A. Rab. Exogenous application of calcium improved the vegetative attributes and corm production in gladiolus. Sarhad Journal of Agriculture 35(3): 1011-1019 (2019).

42. M. Wyszkowski, N. Kordala, and M. S. Brodowska. Trace element content in soils with nitrogen fertilisation and humic acids addition. Agriculture 13(5): 968 (2023).

43. M. Ahmad, W. Iqbal, U. Ahmed, A. Jamal, M.F. Saeed, M.S. Elshikh, M.F. Elsadek, M.A. Ali, J. Černý, and D. Ronga. Enhancing floret persistence and bloom duration in gladiolus through foliar-applied calcium: a sustainable approach to floriculture. The Journal of Horticultural Science and Biotechnology 100(6): 775-788 (2025).

44. X. Wang, J. Zhang, J. Shen, L. Zhang, P. Wei, A. Liu, and H. Song. The alleviating effect on the growth, chlorophyll synthesis, and biochemical defense system in sunflowers under cadmium stress achieved through foliar application of humic acid. BMC Plant Biology 24(1): 792 (2024).

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Published

2025-12-10

How to Cite

Naeem, A., Noor Ul Amin, Ali, H., Ahmad, M., Khattak, A. M., Shafi, A., … Rehman, H. U. (2025). Effect of Humic Acid Levels on the Production of Gladiolus Cultivars. Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, 62(4). https://doi.org/10.53560/PPASB(62-4)1121

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