Extraction and Application of Marigold Dyestuff with (Ocimum tenuiflorum) Oil on Cotton and Polyester Activity against Antibacterial

Application of natural herbs on fabrics


  • Mehreen Ijaz Department of Home Economics, Lahore College for Women University, Lahore, Pakistan
  • Namood-e- Sahar Department of Home Economics, Lahore College for Women University, Lahore, Pakistan
  • Rafia Fatima Department of Home Economics, Lahore College for Women University, Lahore, Pakistan
  • Zohra Tariq Department of Home Economics, Lahore College for Women University, Lahore, Pakistan
  • Hibba Munir Dar Department of Home Economics, Lahore College for Women University, Lahore, Pakistan
  • Madeeha Tariq Department of Home Economics, Lahore College for Women University, Lahore, Pakistan


Antimicrobial, Ocimum tenuiflorum, Marigold, Dyestuff, Cotton, Polyester


A lot of attention has been given by the researchers to seek cheap and natural sources of colorant materials and strategies to improve their fastness against the light, crocking, and washing of textile materials. Marigold flowers were taken to extract dyestuff and applied on the surface of cotton and polyester fabrics to study the colorfastness in dry and wet conditions. Oil was also extracted from leaves of Ocimum tenuiflorum (tulsi) and added in the prepared dye bath to induce antimicrobial properties in the dyed fabrics. Colorfastness of fabrics was evaluated by following AATCC 8-2007 test method. Antimicrobial activity was determined by following recommendations given by AATCC 147-16 test procedure. It was concluded that the scale of colorfastness ranged between 4 and 5 for polyester and cotton fabrics respectively that depict excellent fastness properties. Ocimum tenuiflorum oil helped to reduce the growth of bacteria on treated fabrics. It was found that extracted oil provided satisfactory protection against Staphylococcus aureus and Escherichia coli on both types of fabrics.


S.R.Malpani. Antibacterial treatment on cotton fabric from neem oil, aloe vera and tulsi. International Journal of Advanced Research in Engineering 2: 35-43 (2013).

V. Sivakumar., J. Vijaeeswarri, and J.L. Anna. Effective natural dye extraction from different plant materials using ultrasound. Industrial Crops and Products 33: 116-122 (2011).

N.F. Ali., R.S.R El-Mohamedy, and S. Rajput. Improvement of antimicrobial activity for onion natural dyed fabrics through chitosan pretreatment. Journal of Applied Sciences Research 9: 4993-5002 (2013).

P. Kumari., S.S. Jeet Singh, and N.M. Rose. Eco – Textiles: For Sustainable Development. International Journal of Scientific and Engineering Research 4: 1379-1390 (2013).

R. Singh., A. Jain., S. Panwar., D. Gupta, and S.K. Khare. Antimicrobial activity of some natural dyes. Dyes and Pigments 66: 99-102 (2005).

K.N. Das, and G. Bhattacharya. Use of natural dyes for antimicrobial finish. National Workshop and Seminar on Vegetable Dye and its Application on Textiles 2nd – 4th December, (2011).

E.M. Abdallah. Plants: An alternative source for antimicrobials. Journal of Applied Pharmaceutical Science 1: 16-20 (2011).

V. Viswanad., N.A. Aleykutty., S.M. Zachariah, and V. Prabhakar. Antimicrobial potential of herbal medicines. International Journal of Pharmaceutical Sciences and Research 2: 1651-1655 (2011).

F.A. Nagia and R.S.R El-Mohamedy. Dyeing of wool with natural anthraquinone dyes from Fusarium oxysporum. Dyes and pigments 75: 550-555 (2007).

D.A. Ates, and O. Turgay. Antimicrobial activities of various medicinal and commercial plant extracts. Turkish Journal of Biology 27: 157-162 (2003).

S. Vishwabhan., V.K. Birendra, and S. Vishal. A review on ethnomedical uses of Ocimum sanctum (Tulsi). International Research Journal of Pharmacy 2: 1-3 (2011).

S. Krishna. G. Tulsi-the Wonder Herb (Pharmacological Activities of Ocimum Sanctum). American Journal of Ethnomedicine 1: 89-95. (2014).

S. Zaghloul., A. El-shafie., M. El-bisi, and R. Refaie. Herbal textile finishes–natural antibacterial finishes for cotton fabric. Egyptian Journal of Chemistry 60: 161-180 (2017).

A.P. Pereira. Phenolic compounds and antimicrobial activity of olive (Olea europaea L Cv Cobrancosa) leaves. Molecules 12: 1153-1162 (2007).

K. Nazim., M. Ahmed., and M. Uzair. Growth potential of two species of basil in sandy soil of Karachi. Pakistan journal of Botany, 41: 1637-1644 (2009).

Prefeasibility-study: Cut flower farm (Gladiolus, Marigold, Statice and Chrysanthemum) Small and Medium Enterprise Development Authority Government of Pakistan. (2009)

P. Saha, and S. Dutta. Production of floral dyes from different flowers available in west bengal for textile and dye industry. Norwegian University of Science and Technology: https://folk.ntnu.no/skoge/prost/proceedings/ecce6_sep07/upload/386.pdf (2007).

E. Ghasemi., F. Raofie, and N.M. Najafi. Application of response surface methodology and central composite design for the optimisation of supercritical fluid extraction of essential oils from myrtus communis l . Leaves. Food Chemistry 126: 1449-1453 (2011).

J. Arora., P. Agarwal, and G. Gupta. Rainbow of natural dyes on textiles using plants extracts: Sustainable and eco-friendly processes. Green and Sustainable Chemistry 7: 35-47 (2017).

Bhairi, S. M., & Mohan, C. (2007). Detergents: A guide to the properties and uses of detergents in biology and biochemistry. EMD Biosciences, SanDiego, CA. (2007).

AATCC-8 Colorfastness to crocking, American Association of Textile Chemists and Colorists (2007).

ASTM D-1776 Standard practice for conditioning and testing textiles. West Conshohocken, PA: ASTM International (2010).

AATCC 147 Antimicrobial. Parallel Streak Method, American Association of Textile Chemists and Colorists (2016).

D. Sarkar., K. Mazumdar, and S. Datta. Solvent extraction of yellow colouring matter from natural source-flowers and its appliction on hydrophobic substrates. Man Made Textiles in India 49: 19-25. (2006).

D.B. Patil., K.N. Patil., P.V. Gaikwad., P.J. Patil., U.L. Shewale, and S.B. Bhamburdekar. Extraction of natural dye from rose flower for dyeing cotton fabrics. International Journal for Innovative Research in Multidisciplinary Field 2:135-137 (2016).

Q.Y. Zhu., Q.Y. Zhang, and J. Cao. Cyclodextrin assisted liquid solid extraction for determination of the composition of jujube fruit using ultrahigh performance liquid chromatography with electrochemical detection and quadrupole time of flight tandem mass spectrometry. Food Chemistry 213: 485-493 (2016).

D. Jothi. Extraction of natural dyes from African marigold flower (Tegetes errecta) for textile colouration. Textile Research Journal 8:49-53 (2008).

M. Kumaresan., P.N. Palanisamy, and P.E. Kumar. Application of Eco-friendly Natural dye obtained from flower of Spathodea campanulata on silk using combination of mordants. European Journal of Scientific Research 52: 306-312. (2011).

A.S.M. Raja., P.K. Pareek., D.B. Shakyawar., S.A.Wani., F.A. Nehvi, and A.H. Sofi. Extraction of natural dye from saffron flower waste and its application on pashmina fabric. Advances in Applied Science Research 3: 156-161 (2012).

F. Batool., S. Adeel., M. Azeem., A.A. Khan., I.A. Bhatti., A. Ghaffar, and N. Iqbal. Gamma radiations induced improvement in dyeing properties and colorfastness of cotton fabrics dyed with chicken gizzard leaves extracts. Radiation Physics and Chemistry 89: 33-37. (2013).

G. Mahale, and R.K. Sunanda. Silk dyed with Acalypha (Acalypha wilkesiana) and its fastness. National Institute of Science Communication and Information Resources 1: 15-25 (2003).

C.K. Jha., R. Kumar., V.S. Kumar, and R.V. Devi. Extraction of natural dye from marigold flower (Tagetes erecta l.) and dyeing of fabric and yarns: A focus on colorimetric analysis and fastness properties. Der Pharmacia Lettre 7: 185-195 (2015).

M. Chandra., S. Thiripura., K.R. Senthil, and A. Thiyagarajan. Dyeing of Cotton with Natural Dye Obtained from Flower of Tecoma stans. Universal Journal of Environmental Research & Technology 2: 41-46 (2012).

S.P. Khattak., S. Rafique., T. Hussain., F. Inayat, and B. Ahmad. Colour fastness and tensile strength of cotton fabric dyed with natural extracts of alkanna tinctoria by continuous dyeing technique. Journal of the Chemical Society of Pakistan 37: 903-909 (2015).

D.S.M. AL-Khateeb. Extraction Dyes From Two Natural Plants Olive Leaves and Beta vulgaris And The Uses In dyeing Textile. Journal of Physics: Conference Series 1294: 2109-2145 (2019).

S.R. Shah and B.H. Patel. Characterization and fixation of Ocimum sanctum extract on wool fabric. Bangladesh Textile Today 6: 31-34 (2013).

S. Adeel., S. Ali., I.A. Bhatti, and F. Zsila. Dyeing of cotton fabric using Pomegranate (Punica Granatum) aqueous extract. Asian Journal of Chemistry 21: 3493-3499 (2009).

Sathianarayanan, M. P., Bhat, N. V., Kokate, S. S., & Walunj, V. E. Antibacterial finish for cotton fabric from herbal products. Indian Journal of Fiber and Textile Research 35: 50-58 (2010).

A.A.A. El Aty., G.T. El-Bassyouni, N.A. Abdel-Zaher, and O.W. Guirguis. Experimental study on antimicrobial activity of silk fabric treated with natural dye extract from neem (azadirachta indica) leaves. Fibers and Polymers 19: 1880-1886 (2018).

M. Ijaz., M. Maqsood and M. Naveed. Evaluation of antimicrobial activity of Ocimum tenuiflorum leaves on mechanical behavior of fabrics. Pure and Applied Biology 9: 538-544 (2020).

G. Manokarana and M. Prabakaran. Evaluation of antioxidant and anticorrosion activities of Ligularia fischeri plant extract. Chemical Science and Engineering Research 1:16-25 (2019).

M.K. Swamy, M. S. Akhtar., and U.R. Sinniah. Antimicrobial properties of plant essential oils against human pathogens and their mode of action:an updated review. Evidence-Based Complementary and Alternative Medicine (2016).

Elavarasan, T., Bhakiaraj, D., Jagatheesan, R., Mathavan, M., Kumard, R. S., & Gopalakrishnana, M. Chemical Science and Engineering Research 2:12-20 (2020).

D. Joshi. Experimental study on antimicrobial activity of cotton fabric treated with aloe gel extract aloe Vera plant for controlling the staphylococcus aureus (bacterium). African Journal of Microbiology Research 3:228-232 (2009).

D. Gupta, and A. Laha. Antimicrobial activity of cotton fabric treated with Quercus infectoria extract. National Institute of Science Communication and Information Resources (2007).

A. Calis., G.Y. Celik, and H. Katircioglu. Antimicrobial effect of natural dyes on some pathogenic bacteria. African Journal of Biotechnology 8: 291-293 (2009).

G.K. Balamurugan, and S. Babuskin. Antimicrobial coating of cotton twill tape with neem oil, eucalyptus oil and tulsi oil for medicinal application. Current Science 115: 779-782 (2018).

R. Rajendran., R. Radha., T.M. Kotresh, and E. Csiszar. Development of antimicrobial cotton fabrics using herb loaded nanoparticles. Carbohydrate Polymers 91: 613-17 (2013).




How to Cite

Ijaz, M. ., Sahar, N.- e-., Fatima, R., Tariq, Z., Dar, H. M., & Tariq, M. . (2021). Extraction and Application of Marigold Dyestuff with (Ocimum tenuiflorum) Oil on Cotton and Polyester Activity against Antibacterial: Application of natural herbs on fabrics. Proceedings of the Pakistan Academy of Sciences: Part B (Life and Environmental Sciences), 57(1), 61–68. Retrieved from http://www.ppaspk.org/index.php/PPAS-B/article/view/74